Critical role of an antiviral stress granule containing RIG-I and PKR in viral detection and innate immunity.
Identifieur interne : 001325 ( Main/Exploration ); précédent : 001324; suivant : 001326Critical role of an antiviral stress granule containing RIG-I and PKR in viral detection and innate immunity.
Auteurs : Koji Onomoto [Japon] ; Michihiko Jogi ; Ji-Seung Yoo ; Ryo Narita ; Shiho Morimoto ; Azumi Takemura ; Suryaprakash Sambhara ; Atushi Kawaguchi ; Suguru Osari ; Kyosuke Nagata ; Tomoh Matsumiya ; Hideo Namiki ; Mitsutoshi Yoneyama ; Takashi FujitaSource :
- PloS one [ 1932-6203 ] ; 2012.
Descripteurs français
- KwdFr :
- ARN viral (métabolisme), Animaux, Anticorps antiviraux (immunologie), Cellules HeLa, Cellules Vero, DEAD-box RNA helicases (immunologie), DEAD-box RNA helicases (métabolisme), Fibroblastes, Granulations cytoplasmiques (immunologie), Humains, Immunité innée (immunologie), Immunohistochimie, Infections à Orthomyxoviridae (immunologie), Interféron de type I (immunologie), Protéine-58 à domaine DEAD, Souris, Souris knockout, Virus de la grippe A (immunologie), eIF-2 Kinase (immunologie), eIF-2 Kinase (métabolisme).
- MESH :
- immunologie : Anticorps antiviraux, DEAD-box RNA helicases, Granulations cytoplasmiques, Immunité innée, Infections à Orthomyxoviridae, Interféron de type I, Virus de la grippe A, eIF-2 Kinase.
- métabolisme : ARN viral, DEAD-box RNA helicases, eIF-2 Kinase.
- Animaux, Cellules HeLa, Cellules Vero, Fibroblastes, Humains, Immunohistochimie, Protéine-58 à domaine DEAD, Souris, Souris knockout.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (immunology), Chlorocebus aethiops, Cytoplasmic Granules (immunology), DEAD Box Protein 58, DEAD-box RNA Helicases (immunology), DEAD-box RNA Helicases (metabolism), Fibroblasts, HeLa Cells, Humans, Immunity, Innate (immunology), Immunohistochemistry, Influenza A virus (immunology), Interferon Type I (immunology), Mice, Mice, Knockout, Orthomyxoviridae Infections (immunology), RNA, Viral (metabolism), Vero Cells, eIF-2 Kinase (immunology), eIF-2 Kinase (metabolism).
- MESH :
- chemical , immunology : Antibodies, Viral, DEAD-box RNA Helicases, Interferon Type I, eIF-2 Kinase.
- immunology : Cytoplasmic Granules, Immunity, Innate, Influenza A virus, Orthomyxoviridae Infections.
- chemical , metabolism : DEAD-box RNA Helicases, RNA, Viral, eIF-2 Kinase.
- Animals, Chlorocebus aethiops, DEAD Box Protein 58, Fibroblasts, HeLa Cells, Humans, Immunohistochemistry, Mice, Mice, Knockout, Vero Cells.
Abstract
Retinoic acid inducible gene I (RIG-I)-like receptors (RLRs) function as cytoplasmic sensors for viral RNA to initiate antiviral responses including type I interferon (IFN) production. It has been unclear how RIG-I encounters and senses viral RNA. To address this issue, we examined intracellular localization of RIG-I in response to viral infection using newly generated anti-RIG-I antibody. Immunohistochemical analysis revealed that RLRs localized in virus-induced granules containing stress granule (SG) markers together with viral RNA and antiviral proteins. Because of similarity in morphology and components, we termed these aggregates antiviral stress granules (avSGs). Influenza A virus (IAV) deficient in non-structural protein 1 (NS1) efficiently generated avSGs as well as IFN, however IAV encoding NS1 produced little. Inhibition of avSGs formation by removal of either the SG component or double-stranded RNA (dsRNA)-dependent protein kinase (PKR) resulted in diminished IFN production and concomitant enhancement of viral replication. Furthermore, we observed that transfection of dsRNA resulted in IFN production in an avSGs-dependent manner. These results strongly suggest that the avSG is the locus for non-self RNA sensing and the orchestration of multiple proteins is critical in the triggering of antiviral responses.
DOI: 10.1371/journal.pone.0043031
PubMed: 22912779
Affiliations:
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Onomoto</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Molecular Genetics, Institute for Virus Research, Kyoto University, Kyoto, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Laboratory of Molecular Genetics, Institute for Virus Research, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Jogi, Michihiko" sort="Jogi, Michihiko" uniqKey="Jogi M" first="Michihiko" last="Jogi">Michihiko Jogi</name></author><author><name sortKey="Yoo, Ji Seung" sort="Yoo, Ji Seung" uniqKey="Yoo J" first="Ji-Seung" last="Yoo">Ji-Seung Yoo</name></author><author><name sortKey="Narita, Ryo" sort="Narita, Ryo" uniqKey="Narita R" first="Ryo" last="Narita">Ryo Narita</name></author><author><name sortKey="Morimoto, Shiho" sort="Morimoto, Shiho" 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Cells</term><term>Humans</term><term>Immunity, Innate (immunology)</term><term>Immunohistochemistry</term><term>Influenza A virus (immunology)</term><term>Interferon Type I (immunology)</term><term>Mice</term><term>Mice, Knockout</term><term>Orthomyxoviridae Infections (immunology)</term><term>RNA, Viral (metabolism)</term><term>Vero Cells</term><term>eIF-2 Kinase (immunology)</term><term>eIF-2 Kinase (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (métabolisme)</term><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Cellules HeLa</term><term>Cellules Vero</term><term>DEAD-box RNA helicases (immunologie)</term><term>DEAD-box RNA helicases (métabolisme)</term><term>Fibroblastes</term><term>Granulations cytoplasmiques (immunologie)</term><term>Humains</term><term>Immunité innée (immunologie)</term><term>Immunohistochimie</term><term>Infections à Orthomyxoviridae (immunologie)</term><term>Interféron de type I (immunologie)</term><term>Protéine-58 à domaine DEAD</term><term>Souris</term><term>Souris knockout</term><term>Virus de la grippe A (immunologie)</term><term>eIF-2 Kinase (immunologie)</term><term>eIF-2 Kinase (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>DEAD-box RNA Helicases</term><term>Interferon Type I</term><term>eIF-2 Kinase</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>DEAD-box RNA helicases</term><term>Granulations cytoplasmiques</term><term>Immunité innée</term><term>Infections à Orthomyxoviridae</term><term>Interféron de type I</term><term>Virus de la grippe A</term><term>eIF-2 Kinase</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Cytoplasmic Granules</term><term>Immunity, Innate</term><term>Influenza A virus</term><term>Orthomyxoviridae 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type="abstract" xml:lang="en">Retinoic acid inducible gene I (RIG-I)-like receptors (RLRs) function as cytoplasmic sensors for viral RNA to initiate antiviral responses including type I interferon (IFN) production. It has been unclear how RIG-I encounters and senses viral RNA. To address this issue, we examined intracellular localization of RIG-I in response to viral infection using newly generated anti-RIG-I antibody. Immunohistochemical analysis revealed that RLRs localized in virus-induced granules containing stress granule (SG) markers together with viral RNA and antiviral proteins. Because of similarity in morphology and components, we termed these aggregates antiviral stress granules (avSGs). Influenza A virus (IAV) deficient in non-structural protein 1 (NS1) efficiently generated avSGs as well as IFN, however IAV encoding NS1 produced little. Inhibition of avSGs formation by removal of either the SG component or double-stranded RNA (dsRNA)-dependent protein kinase (PKR) resulted in diminished IFN production and concomitant enhancement of viral replication. Furthermore, we observed that transfection of dsRNA resulted in IFN production in an avSGs-dependent manner. These results strongly suggest that the avSG is the locus for non-self RNA sensing and the orchestration of multiple proteins is critical in the triggering of antiviral responses.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><noCountry><name sortKey="Fujita, Takashi" sort="Fujita, Takashi" uniqKey="Fujita T" first="Takashi" last="Fujita">Takashi Fujita</name><name sortKey="Jogi, Michihiko" sort="Jogi, Michihiko" uniqKey="Jogi M" first="Michihiko" last="Jogi">Michihiko Jogi</name><name sortKey="Kawaguchi, Atushi" sort="Kawaguchi, Atushi" uniqKey="Kawaguchi A" first="Atushi" last="Kawaguchi">Atushi Kawaguchi</name><name sortKey="Matsumiya, Tomoh" sort="Matsumiya, Tomoh" uniqKey="Matsumiya T" first="Tomoh" last="Matsumiya">Tomoh Matsumiya</name><name sortKey="Morimoto, Shiho" sort="Morimoto, Shiho" uniqKey="Morimoto S" first="Shiho" last="Morimoto">Shiho Morimoto</name><name sortKey="Nagata, Kyosuke" sort="Nagata, Kyosuke" uniqKey="Nagata K" first="Kyosuke" last="Nagata">Kyosuke Nagata</name><name sortKey="Namiki, Hideo" sort="Namiki, Hideo" uniqKey="Namiki H" first="Hideo" last="Namiki">Hideo Namiki</name><name sortKey="Narita, Ryo" sort="Narita, Ryo" uniqKey="Narita R" first="Ryo" last="Narita">Ryo Narita</name><name sortKey="Osari, Suguru" sort="Osari, Suguru" uniqKey="Osari S" first="Suguru" last="Osari">Suguru Osari</name><name sortKey="Sambhara, Suryaprakash" sort="Sambhara, Suryaprakash" uniqKey="Sambhara S" first="Suryaprakash" last="Sambhara">Suryaprakash Sambhara</name><name sortKey="Takemura, Azumi" sort="Takemura, Azumi" uniqKey="Takemura A" first="Azumi" last="Takemura">Azumi Takemura</name><name sortKey="Yoneyama, Mitsutoshi" sort="Yoneyama, Mitsutoshi" uniqKey="Yoneyama M" first="Mitsutoshi" last="Yoneyama">Mitsutoshi Yoneyama</name><name sortKey="Yoo, Ji Seung" sort="Yoo, Ji Seung" uniqKey="Yoo J" first="Ji-Seung" last="Yoo">Ji-Seung Yoo</name></noCountry><country name="Japon"><region name="Région du Kansai"><name sortKey="Onomoto, Koji" sort="Onomoto, Koji" uniqKey="Onomoto K" first="Koji" last="Onomoto">Koji Onomoto</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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